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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク DNA nanoball sequencing ： ウィキペディア英語版 DNA nanoball sequencing DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses rolling circle replication to amplify small fragments of genomic DNA into ''DNA nanoballs''. Fluorescent probes bind to complementary DNA and the probes are then ligated to anchor sequences bound to known sequences on the DNA template. The base order is determined via the fluorescence of the ligated and bound probes. This DNA sequencing method allows large numbers of DNA nanoballs to be sequenced per run at lower reagent costs compared to other next generation sequencing platforms. However, a limitation of this method is that it generates only short sequences of DNA, which presents challenges to mapping its reads to a reference genome.〔 The company Complete Genomics uses ''DNA nanoball sequencing'' to sequence samples submitted by researchers. ==Procedure== DNA Nanoball Sequencing involves isolating DNA that is to be sequenced, shearing it into small 400 – 500 base pair (bp) fragments, ligating adapter sequences to the fragments, and circularizing the fragments. The circular fragments are copied by rolling circle replication resulting in many single-stranded copies of each fragment. The DNA copies concatenate head to tail in a long strand, and are compacted into a DNA nanoball. The nanoballs are then adsorbed onto a sequencing flow-cell. Unchained sequencing reactions interrogate specific nucleotide locations in the nanoball by ligating fluorescent probes to the DNA. The color of the fluorescence at each interrogated position is recorded through a high-resolution camera. Bioinformatics are used to analyze the fluorescence data and make a base call, and for mapping the 35-bp mate pair reads to a reference genome. The genome is assembled and any polymorphisms present in the sequence are identified.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「DNA nanoball sequencing」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.